Method of assay for antigen
US5236830A · kind A · utility
Inventor
Key dates
| Filing date | Nov 3, 1989 |
| Grant date | Aug 17, 1993 |
| Priority date | — |
| Expiry date | Nov 3, 2009 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/822
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method of assay for antigen comprising the following sequential steps (A), (B), (C) and (D): PA0 (A): the antigen to be assayed in a subject solution is bound with a functional group or marker to form a modified antigen; PA0 (B): the modified antigen is bound to a carrier via an antibody against the antigen, and then the carrier is separated from the subject solution; PA0 (C): Either (a) or (b): PA1 (a) the modified antigen is dissociated from the carrier; or PA1 (b) the modified antigen-antibody complex comprising the modified antigen and the antibody against the antigen is dissociated from the carrier; and PA0 (D): the modified antigen or modified antigen-antibody complex of Step (C) is assayed. This method permits assay for antigens with high sensitivity based on characteristic features of the sandwich method, using an antibody against a single epitopic site, and also permits assay for low molecular substances, which have never been assayed by the conventional sandwich method, with higher sensitivity in comparison with the conventional competitive method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.