Electro insertion of proteins into red cell membranes
US5236835A · kind A · utility
6Cited by
0References
16Claims
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Key dates
| Filing date | Dec 17, 1991 |
| Grant date | Aug 17, 1993 |
| Priority date | — |
| Expiry date | Dec 17, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N5/0006
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of incorporating human CD4 or glycophorin into a red blood cell membrane is provided comprising exposing the red blood cell to an electric field while the cell is suspended in an electro-insertion medium in the presence of a buffered solution (suspension) of the proteins. These proteins bear a hydrophobic membrane spanning sequence. The resultant cell of the electro-insertion is then contacted with a resealing medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.