Patent · US Expired

Enzymatic reagent for D-malate determination

US5236837A · kind A · utility

3Cited by

7References

9Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Hans-Otto Beutler · Tutzing, DE
Gunter Lang · Wernau, DE
Klaus Kaluza · Bad Heilbrunn, DE

Key dates

Filing date	Aug 9, 1990
Grant date	Aug 17, 1993
Priority date	—
Expiry date	Aug 9, 2010

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S435/81
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

The invention concerns a new NAD.sup.+ -dependent D-malate dehydrogenase (decarboxylating, E.C. 1.1.1.83) from E. coli which consists of two subunits which each have a molecular weight of about 42 kD (SDS gel) and processes for the isolation of this enzyme from E. coli and for its stabilisation. The invention concerns in addition a method for the determination of D-malate with the D-malate dehydrogenase according to the present invention as well as a reagent for determining D-malate.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.