End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
US5237016A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 5, 1989 |
| Grant date | Aug 17, 1993 |
| Priority date | — |
| Expiry date | Jan 5, 2009 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
This invention concerns methods and means for covalent attachment of oligonucleotides to solid supports substantially at their 5'-ends. According to the invention thiol-oligonucleotides are attached to bromoacetyl-derivatized polyacrylamide supports, or conversely, bromoacetyl-oligonucleotides are immobilized on thiol-polyacrylamide supports. In a further aspect, this invention relates to bromoacetyl-oligonucleotides that may be immobilized on thiol-polyacrylamide solid supports, thiol-oligonucleotides immobilized on bromoacetyl-derivatized polyacrylamide supports as well as to methods for capture of nucleic acids by oligonucleotides attached to polyacrylamide solid supports, either by direct capture or in sandwich hybridization formats.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.