Method of determining energy transfer efficiency of a donor-acceptor pair of fluorescent dyes
US5237515A · kind A · utility
31Cited by
3References
3Claims
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Key dates
| Filing date | Apr 10, 1991 |
| Grant date | Aug 17, 1993 |
| Priority date | — |
| Expiry date | Apr 10, 2011 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2201/129
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The energy transfer efficiency of a donor-acceptor pair of fluorescent dyes can be determined by first measuring the fluorescence and absorption spectra of donor-protein, acceptor-protein, mixture of donor-protein and acceptor-protein, and donor-acceptor-protein conjugates, then separating the respective spectra into their respective donor-protein complex and acceptor-protein complex components using multiple linear regression, and then determining the transfer efficiency on the basis of the quenching of the donor fluorescence based on the spectral data thus obtained.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.