Molecular cloning and expression in industrial Bacillus species
US5238833A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | May 13, 1991 |
| Grant date | Aug 24, 1993 |
| Priority date | — |
| Expiry date | May 13, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/75
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Novel methods and novel industrial unicellular microorganism strains, particularly industrial Bacillus strains, are provided for enhanced production of endogenous and exogenous polypeptides. Cloning vehicles containing the gene expressing the polypeptide of interest are introduced into a compatible host. Transformed hosts harboring the introduced vehicle in a stable way by integration of the vehicle into the host cells chromosome are selected Efficient transfer of the vehicle containing the gene of interest is achieved, with the resulting industrial strain transformants being effective, stable producers of the desired polypeptide product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.