Patent · US Expired

Process to induce desiccation tolerance in somatic embryos

US5238835A · kind A · utility

27Cited by
3References
17Claims
0Family size

Assignee

Inventors

Key dates

Filing dateOct 19, 1990
Grant dateAug 24, 1993
Priority date
Expiry dateOct 19, 2010

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S47/09
  • WIPO fieldFood chemistry
  • WIPO sectorChemistry

Abstract

A process for inducing desiccation tolerance in an in vitro formed plant embryo to enable production of viable artificial seeds is disclosed. The process comprises culturing in vitro plant embryos, stimulating the embryos to proceed through globular and a heart shaped stage into an elongate-torpedo shaped stage and early cotyledon stage, inducing the embryos as early as the torpedo-shaped stage with a source of abscisic acid at an effective concentration of abscisic acid and for a sufficient period of time to cause expression of desiccation tolerance which includes change in cellular metabolism, electron transport processes and oxidation-reduction reactions in the embryos and drying the induced embryos to provide stable viable embryos.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.