Recombinant vector having a Streptomyces achromogenes DNA sequence useful for gene amplification
US5240858A · kind A · utility
1Cited by
0References
3Claims
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Assignee
Inventors
Key dates
| Filing date | Jan 8, 1991 |
| Grant date | Aug 31, 1993 |
| Priority date | — |
| Expiry date | Jan 8, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/74
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An improved means for amplifying DNA sequences is disclosed. The invention provides a means for creating tandemly repeated gene sequences. One attaches a selected foreign gene sequence in a S. achromogenes fragment and then exposes the recombinant segment to antibiotic challenge. Temperature sensitive plasmids or other curable vectors can be used to assist in the amplification and the positioning of the resulting gene directly in chromosomal host DNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.