Method of preparing angiotensin converting enzyme inhibitors
US5243027A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 31, 1991 |
| Grant date | Sep 7, 1993 |
| Priority date | — |
| Expiry date | Jul 31, 2011 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of preparing an angiotensin converting enzyme inhibitor having the amino acid sequence selected from the group consisting of: PA0 Gly-Lys-Glu-Ile-Ile-Val-Lys-Ala-Glu-Arg (SEQ ID No: 1), PA0 Gly-Lys-Met-Val-Lys-Val-Val-Ser-Trp-Tyr (SEQ ID No: 2) and PA0 Ala-Tyr-Ile-Ala-Ser-Lys-Gly-Leu (SEQ ID No: 3); which comprises the steps of: PA1 (a) disrupting bacteria to obtain a cell extract, wherein the disruption is at least one of mechanical and chemical disruption; PA1 (b) exposing said cell extract to an acid mixture comprising acetic acid and hydrochloric acid, wherein said acid mixture comprises acetic acid in a concentration of 0.01 to 10M and hydrochloric acid in a concentration of 1 to 100 mM, to produce an acid hydrolysate; and PA1 (c) purifying from said acid hydrolysate a peptide having the amino acid sequence selected from the group consisting of: PA2 Gly-Lys-Glu-Ile-Ile-Val-Lys-Ala-Glu-Arg (SEQ ID No: 1), PA2 Gly-Lys-Met-Val-Lys-Val-Val-Ser-Trp-Tyr (SEQ ID No: 2) and PA2 Ala-Tyr-Ile-Ala-Ser-Lys-Gly-Leu (SEQ ID No: 3).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.