Determination of high molecular weight analytes using a .beta.-galactosidase complementation assay
US5244785A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Feb 1, 1991 |
| Grant date | Sep 14, 1993 |
| Priority date | — |
| Expiry date | Feb 1, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for detecting the presence of a high-molecular-weight analyte, which uses a variation on complementation assays, comprising (1) providing an enzyme donor (ED)complex comprising ED coupled to an analyte-specific binding molecule, wherein the ED complex retains measurable complementation activity such that active .beta.-galactosidase is formed in the presence of an enzyme acceptor (EA); (2) contacting the ED complex and EA in the presence of a sample suspected of containing a high-molecular-weight analyte that reacts specifically with the analyte-specific binding molecule; and (3) relating the presence of the analyte in the sample to the formation of active .beta.-galactosidase enzyme.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.