Cloned genes encoding reverse transcriptase lacking RNase H activity
US5244797A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Mar 18, 1991 |
| Grant date | Sep 14, 1993 |
| Priority date | — |
| Expiry date | Mar 18, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/975
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.