Thiobenzoylation method of peptide sequencing with gas chromatography and mass spectrometric detection
US5246865A · kind A · utility
10Cited by
6References
18Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jul 24, 1992 |
| Grant date | Sep 21, 1993 |
| Priority date | — |
| Expiry date | Jul 24, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/24
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A protein microsequencing method for use in conjunction with the thiobenzoylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thiobenzoylating reagent. The derivatized polypeptide is subjected to cleavage by acid which forms a 4-substituted 2-phenyl-5(4H) thiazolone. The thiazolone is acylated to form a 5-acyloxy-2-phenylthiazole and subjected to detection by both gas chromatography and chemical ionization mass spectroscopy.
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