Determination and quantification of saccharides by luminescence lifetimes and energy transfer
US5246867A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Jan 17, 1992 |
| Grant date | Sep 21, 1993 |
| Priority date | — |
| Expiry date | Jan 17, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/144444
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method for measuring the concentration of a saccharide, conjugated saccharide or polysaccharide of interest using luminescent lifetimes and energy transfer in which an energy transfer donor-acceptor pair is added to a sample to be analyzed, the donor of the donor-acceptor pair being photoluminescent. The acceptor is bound to a carrier, while the donor and any saccharide, conjugated saccharide or polysaccharide of interest present in the sample compete for binding sites on the carrier. The sample is irradiated and the resultant emission detected. Energy transfer occurs between the donors and the acceptors, which produces a detectable lifetime change of the fIuorescence of the donor. The lifetime change is reduced or even eliminated by the competitive binding of a saccharide, conjugated saccharide or polysaccharide of interest to the donor. By measuring the apparent luminescent lifetime, the amount of a saccharide, conjugated saccharide or polysaccharide of interest in the sample can be determined.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.