Patent · US Expired

Use of a derivatized alkaline phosphatase as a standard

US5248592A · kind A · utility

2Cited by

2References

7Claims

0Family size

Assignee

Boehringer Mannheim GmbH · DE

Inventors

Wilhelm Tischer · Peißenberg, DE
Martin T. Gerber · Maple Grove, US
Hellmuth Vetter · Tutzing, DE

Key dates

Filing date	Apr 16, 1992
Grant date	Sep 28, 1993
Priority date	—
Expiry date	Apr 16, 2012

Classification

Technology area (CPC Y)Emerging Cross-Sectional Technologies
CPC primaryY10S436/827
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

Alkaline phosphatase to which is covalently bound a carbohydrate of the general formula ##STR1## wherein n is 0, 1, 2 or 3, A is an acyl radical containing 2 to 5 carbon atoms, R.sup.1 and R.sup.2 are each hydrogen atoms or hydroxyl groups, R.sup.3 is --COOH or --CH.sub.2 OH, R.sup.4 is a hydroxyl group or a --CHOH--CHOH--CH.sub.2 OH or --NH--CO--CH.sub.2 --CH(NH.sub.2)--COOH radical or a complex containing the carbohydrate, is used as a standard for the determination of human alkaline phosphate. Preferred carbohydrates are ovomucoid, chitobiose, chitotriose or N-acetylglucosamine-N-acetylneuraminic acid.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.