Reagents, methods and kits for an amphetamine-class fluorescence polarization immunoassay
US5248791A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 14, 1992 |
| Grant date | Sep 28, 1993 |
| Priority date | — |
| Expiry date | Jan 14, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/946
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A fluorescence polarization immunoassay (FPIA) for detecting the presence of one or more amphetamine-class analytes in a test sample is provided. The immunoassay uses competition between the analyte and a fluorescently labeled tracer for the binding site on an antibody specific for phenethylamine derivatives. The concentration of amphetamine-class analyte in the sample determines the amount of tracer that binds to the antibody. The amount of tracer/antibody complex formed can be quantitatively measured and is inversely proportional to the quantity of analyte in the test sample. The invention relates to tracers, to immunogens used to elicit antibodies for use as assay reagents, and to assay kits incorporating these tracers and assay reagents.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.