HPLC light scattering detector for biopolymers
US5250186A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Oct 9, 1992 |
| Grant date | Oct 5, 1993 |
| Priority date | — |
| Expiry date | Oct 9, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/25375
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A high angle light scattering detector using classical Rayleigh scattering. A high intensity arc light source, filtered to leave only one wavelength illuminates a flow cell. Through the flow cell, very small particles such as biological proteins flow in solution after separation by HPLC or some other means. A UV detector generates data regarding the weight concentration of the eluting particles and a scattered light detector collecting scattered light at angles of approximately 90.degree. generates a scattered light signal. The incident light intensity is also measured. The average molecular weight is then computed using the scattered and incident light data, the weight concentration data and a simplified mathematical relationship from which the size factor P and the viral coefficients have been eliminated.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.