Process for enzymatic ultrafiltration of deamidated protein
US5250305A · kind A · utility
7Cited by
2References
9Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 27, 1990 |
| Grant date | Oct 5, 1993 |
| Priority date | — |
| Expiry date | Jun 27, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y305/01043
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention relates to methods for the large-scale enzymatic deamidation of foot proteins. Deamidating enzymes such as peptidoglutaminase are immobilized using a spiral membrane having a molecular weight cut-off, from about 3 to about 50 kd. The methods minimize loss of the enzymatic activity for subsequent reuse of the enzyme as well as increase deamidation product yield.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.