Method for acrosome reaction assay
US5250417A · kind A · utility
8Cited by
1References
24Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 23, 1991 |
| Grant date | Oct 5, 1993 |
| Priority date | — |
| Expiry date | Apr 23, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/906
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method is provided for rapidly determining the ability of sperm to undergo the acrosome reaction, using fluorescence microscopy, light microscopy or color change on a dot blot assay to determine the relative percentage of acrosome reacted sperm. Sperm are capacitated through use of bromelain or other enzymatic substances capable of capacitating sperm, with the total incubation time required to induce capacitation as little as fifteen minutes, yielding sperm that are fully capacitated and show excellent motility, with as many as 80% of normal sperm consistently induced to acrosome react.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.