Reagent for simultaneous determination of hemoglobin and leukocytes in blood
US5250437A · kind A · utility
36Cited by
13References
7Claims
0Family size
Assignee
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Key dates
| Filing date | Oct 10, 1990 |
| Grant date | Oct 5, 1993 |
| Priority date | — |
| Expiry date | Oct 10, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/108331
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A reagent for measuring the hemoglobin concentration and counting the number of leukocytes in a blood sample, which contains at least one of a quaternary ammonium salt or a pyridinium salt, having a concentration capable of hemolyzing erythrocytes in the blood and denaturing hemoglobin and at least one of cationic, nonionic or amphoteric surfactants or an oxidant capable of oxidizing heme in hemoglobin, the reagent being capable of dividing leukocytes into two or three fractions, for example, a fraction of lymphocytes, a fraction of monocytes, eosinophils and basophils, and a fraction of neutrophils.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.