Method for differential determination of white blood cells using diazolidinyl urea to stabilize white blood cells
US5250438A · kind A · utility
35Cited by
10References
7Claims
0Family size
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Key dates
| Filing date | May 9, 1990 |
| Grant date | Oct 5, 1993 |
| Priority date | — |
| Expiry date | May 9, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/108331
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A new method for separation of white cells in differential hematology instruments is described. In the method described, white cell shrinkage is controlled by the use of diazolidinyl urea and accelerated with alcohol thereby providing a reproducible method for easy manipulation of the white cells. In addition, it was found that adsorption of blood platelets and red blood cells in the instruments can be prevented by use of a surfactant such as the polyols.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.