Enzyme activity determinations method characterized by the using of substrates whose fluorescent properties differs those of the converted products

US5252462A · kind A · utility

Assignees

• Pharmacia AB · SE
• WALLAC OY · FI

Inventors

• Hakan N. Y. Drevin · Uppsala, SE
• ANNA MARTIN · Uppsala, SE
• Jan P. E. Carlsson · Uppsala, SE
• Sven Oscarsson · Örbyhus, SE
• Timo Lovgren · Turku, FI
• Ilkka Hemmila · Turku, FI
• Marek Kwiatkowski · Uppsala, SE

Key dates

• Filing date: Jan 5, 1990
• Grant date: Oct 12, 1993
• Expiry date: Jan 5, 2010

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S436/80
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The method for demonstrating the presence of an activity of an enzyme by PA1 (a) incubating said enzyme with a fluorogenic substrate A which is converted by the enzyme to a product B differing from A in respect to its fluorescent properties, A and/or B carrying a chromophore which is a triplet sensitizer having a triplet energy level above the excitation energy level of a lanthanide ion selected from the group consisting of Eu.sup.3+, Tb.sup.3+, Dy.sup.3+ and Sm.sup.3+ and which is capable of chelating said lanthanide ion by means of an oxygen or nitrogen atom in said chromophore, and that B differs from A either by PA2 (i) carrying a different chromophore, or PA2 (ii) having a different chelating ability, and PA1 (b) measuring the change in fluorescence caused by said enzyme.