Clipsin, a chymotrypsin-like protease and method of using same
US5252463A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 22, 1990 |
| Grant date | Oct 12, 1993 |
| Priority date | — |
| Expiry date | Jun 22, 2010 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2800/2821
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A novel M.sub.r 25,000 chymotrypsin-like protease, termed clipsin, which binds to a-1-AChy in a sodium dodecyl sulfate-resistant manner, preferentially degrades .beta.-APP, and shows high activity for a period of a few days in neonatal rat brain is disclosed. Preparation of clipsin in a form that allows clipsin proteolytic activity to be measured under conditions substantially free of the activity other cellular non-clipsin proteases is also disclosed. Further disclosed is a method of identifying potential therapeutic agents for the treatment of selected neuropathologies such as Down's syndrome and Alzheimer's disease by measuring or inhibition of clipsin.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.