Flourescence intramolecular energy transfer conjugate compositions and detection methods
US5254477A · kind A · utility
197Cited by
1References
4Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Sep 19, 1991 |
| Grant date | Oct 19, 1993 |
| Priority date | — |
| Expiry date | Sep 19, 2011 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/144444
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A variety of conjugated compositions and methods for the detection of an analyte of interest in a fluid sample is provided which relies upon an intramolecular energy transfer between a conjugated fluorophore and a chromophoric light-absorbing ligand for qualitative and quantitative results. The detection methods preferably employ fiber optic sensors in combination with analyte-insensitive fluorophores and analyte-sensitive absorber ligands in conjugated form. The methods and compositions rely upon the ability of the absorbing ligands to absorb energy which is transferred non-radiatively by the fluorophore when in an excited state.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.