Hybridization assay and means to be used in the assay
US5256535A · kind A · utility
Inventors
Key dates
| Filing date | Jun 16, 1988 |
| Grant date | Oct 26, 1993 |
| Priority date | — |
| Expiry date | Jun 16, 2008 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A method for the detection of a nucleotide sequence of a nucleic acid in a sample. The method comprises the steps: (i) contacting under hybridization condition the single stranded form of the nucleotide sequence with a single stranded nucleic acid probe, in which plurality of rare earth metal chelate groups is covalently linked via a water-soluble polymer of non-nucleic acid structure to a nucleotide acid that as one of its strand has the nucleotide sequence to be detected and as the other strand the nucleotide sequence of the probe, and (ii) detecting the formation of double stranded nucleic acid. The plurality of rare earth metal chelate groups have at least one metal ion selected from the group consisting of Eu.sup.3+, Sm.sup.3+, Tb.sup.3+ and Dy.sup.3+ as the chelated rare earth metal. The probes as given above are also claimed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.