Expression of human immunodeficiency virus (HIV) reverse transcriptase

US5256554A · kind A · utility

Assignees

• The Trustees of Columbia University in the City of New York · US
• DANA-FARBER CANCER INSTITUTE, INC. · US

Inventors

• Stephen P. Goff · Orefield, US
• Naoko Tanese · New York, US
• William A. Haseltine · Cambridge, US

Key dates

• Filing date: Dec 2, 1991
• Grant date: Oct 26, 1993
• Expiry date: Dec 2, 2011

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S930/221
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

This invention describes pHRT25, a plasmid containing a modified pol gene of the Human Immunodeficiency Virus Type 1 (HIV-1), formerly HTLV-III, under control of an inducible trp promoter. Methods of expressing reverse transcriptase activity using pHRT25 in E. coli are described.