Compositions and methods for increasing the yields of in vitro RNA transcription and other polynucleotide synthetic reactions
US5256555A · kind A · utility
46Cited by
1References
9Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 20, 1991 |
| Grant date | Oct 26, 1993 |
| Priority date | — |
| Expiry date | Dec 20, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/34
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to compositions and methods for increasing the yields of polynucleotide synthetic reactions. In particular, it relates to an improved reaction mixture for use in in vitro RNA transcription and in various other enzymatic reactions in which a polynucleotide is synthesized. The reaction mixture uses high concentrations of total nucleotides, in the order of 12 mM to 40 mM, i.e. levels that were previously thought to be inhibitory. Other useful modifications are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.