Process for obtaining invertase from yeast

US5256556A · kind A · utility

Assignee

• Gesellschaft fur Biotechnologische Forschung mbH (GBF) · DE

Inventors

• Helmut Hustedt · Braunschweig, DE
• Kay Buntemeyer · Braunschweig, DE
• Karl-Heinz Kroner · Braunschweig, DE
• Bernhard Borner · Alzenau, DE

Key dates

• Filing date: Aug 13, 1990
• Grant date: Oct 26, 1993
• Expiry date: Aug 13, 2010

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/814
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Invertase is produced by a process wherein, first the yeast cells are disrupted to produce a disrupted cell suspension, second the disrupted cell suspension is adjusted to an acidic pH, third denatured undersired proteins are removed with the cell detritus and lastly the invertase is isolated. The improvement of the invention to the state of the art is that it comprises subjecting the disrupted cell suspension prior to removing the undesired proteins and cell detritus, to a pH of less than 4.5 and to a heat treatment in a continuous thermal denaturation system at a temperature in a range of from 44.degree. C. to about 51.degree. C.