Patent · US Expired

Purified alkaline protease concentrate and method of preparation

US5256557A · kind A · utility

19Cited by
3References
8Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 27, 1991
Grant dateOct 26, 1993
Priority date
Expiry dateDec 27, 2011

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY10S435/816
  • WIPO fieldBasic materials chemistry
  • WIPO sectorChemistry

Abstract

A highly purified alkaline protease preparation is produced by separating a mixture containing amorphous form and crystalline form of alkaline protease from ultrafiltrate containing impurities, through a method which includes the incubation of an aqueous alkaline protease concentrate with sodium chloride and carbohydrate hydrolyse enzymes at an elevated temperature greater than 20.degree. C. and a pH between pH 4.0 and pH 6.5 at constant agitation.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.