Fluorophore-assisted cloning analysis
US5258295A · kind A · utility
14Cited by
1References
13Claims
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Assignee
Inventors
Key dates
| Filing date | Feb 25, 1992 |
| Grant date | Nov 2, 1993 |
| Priority date | — |
| Expiry date | Feb 25, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y302/01023
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Various assays for the detection and quantitation of carbohydrate-interacting proteins are disclosed. Carbohydrate-interacting proteins include carbohydrate modifying enzymes and carbohydrate binding proteins. The assays employ a fluorophore-labelled carbohydrate substrate that either is capable of being structurally modified by the carbohydrate-modifying enzyme being assayed or changes its electrophoretic gel migration tube upon interaction with a carbohydrate-binding protein. Methods for cloning genes encoding carbohydrate-modifying enzymes are also disclosed.
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