Single stage avian embryo culture process
US5258307A · kind A · utility
2Cited by
0References
4Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Sep 17, 1990 |
| Grant date | Nov 2, 1993 |
| Priority date | — |
| Expiry date | Sep 17, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2500/60
- WIPO fieldOther special machines
- WIPO sectorMechanical engineering
Abstract
A novel single stage culture system for avian embryos is disclosed. Premature eggs consisting of about 32 to about 100 cells are collected shortly after ovulation and prepared for culture. The eggs are incubated in a potassium rich bicarbonate solution and the contents of the eggs are transferred to recipient eggshells. The open end of the eggshell is closed and the embryos are turned and incubated in an oxygen enriched atmosphere for about 3-4 days. For the final incubation period the eggs are incubated in an upright position and rocked hourly.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.