Peptide synthesis method and solid support for use in the method

US5258454A · kind A · utility

Assignee

• Riso National Laboratory · DK

Inventors

• Rolf Henrik Berg · Hørsholm, DK
• Kristoffer Almdal · Kiel, DE
• Walther B. Pedersen · Roskilde, DK
• Arne Holm · Paseo Los Muchachos, DK
• James P. Tam · New York, US
• Robert B. Merrifield · Cresskill, US

Key dates

• Filing date: May 12, 1992
• Grant date: Nov 2, 1993
• Expiry date: May 12, 2012

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K1/042
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for the solid-phase synthesis of peptides or proteins in high yield and high purity uses a solid-support consisting of a functionalized polystyrene-grafted polymer substrate, the grafted polystyrene chains being substantially non-cross-linked and having a chain molecular weight, not including optional non-reactive substituents, of at least 200,000, preferably in the range of 600,000-1,200,000. Particularly suitable polymer substrates are substrates of a polyolefin such as polyethylene. The method is particularly well-suited to the compartmentalized synthesis of a multitude of peptides or proteins in a parallel and substantially simultaneous fashion. Preferred embodiments of a solid support for performing the synthesis are prepared from thin polyethylene sheet or film which has been grafted with polystyrene chains in a radical-initiated process in which the polyethylene sheet or film is immersed in a solution of optionally substituted styrene monomer in an alcohol such as methanol, the volume percentage of styrene in the solution preferably being about 30% v/v, and subjected to gamma irradiation.