Process for purifying annexines
US5258497A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jul 13, 1990 |
| Grant date | Nov 2, 1993 |
| Priority date | — |
| Expiry date | Jul 13, 2010 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/4721
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to processes for purifying annexines. The overall process includes the steps of preparing a homogenized cell preparation and adjusting the pH to about 8.0 to 10.0, adding at least one bivalent cation, adding a phospholipid, washing the insoluble cell residue to remove the soluble constituents, and extracting the annexines from the cell residue with a chelating agent. This process initially promotes the adsorption of the annexines onto the insoluble cell residue or membrane of the host organism. The adsorption step makes it possible to eliminate unwanted soluble matter from the homogenized material by simple washing. Desorption is accomplished by using a chelating agent.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.