Calibrated pipette tip and method
US5260030A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | Jun 3, 1992 |
| Grant date | Nov 9, 1993 |
| Priority date | — |
| Expiry date | Jun 3, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2035/1062
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A pipette tip (30) for use with a pipetter (10) and a method for dispensing a precise volume of a fluid (54). The pipette tip (30) has a hollow body (32), which defines an interior volume (34), and a channel (42) therein having a calibrated volume. Channel (42) extends from an intake opening (44) to an overflow opening (46) positioned inside the interior volume (34) of the tip above a reservoir portion (50) inside the tip. Fluid (54) drawn through the intake opening (44) and into the channel (42) in excess of the calibrated channel volume is gravity-shed or sloughed into an overflow reservoir (50). Thus, a calibrated volume of fluid (54) remains in the channel (42) and can be dispensed accurately from the channel (42). Additionally, the reservoir portion (50) captures and contains the overflow fluid in a stable condition, permitting the reservoir (50) to be used to react various substances (76, 77) with the overflow fluid (56a) inside the pipette tip (30). The internal reservoir (50) also may be divided by a partition (72) to provide a plurality of reservoirs or sub-volumes (73, 74) inside the pipette tip (30).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.