Tissue fixative solution and method
US5260048A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 4, 1992 |
| Grant date | Nov 9, 1993 |
| Priority date | — |
| Expiry date | May 4, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2474/20
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A tissue fixative solution and method is disclosed which comprises a histological fixing composition of diazolidinyl urea, 2-bromo-2-nitropropane-1,3-diol and a water-soluble zinc salt such as zinc sulfate dissolved in a solvent such as water, alcohol or dimethylsulfoxide. The fixative solution of the invention is much less toxic than prior art fixatives and thus can be used more safely, conveniently and effectively than previous solutions in histological techniques. In addition, the fixative solution allows good tissue preservation to be obtained, and because tissue antigens are retained, the fixative is useful for all types of immunostaining procedures.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.