Nonaqueous polymeric reagent compositions and applications thereof

US5260195A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Abol Azhar · Fishers, US
• Arthur M. Usmani · Indianapolis, US
• Anthony Burke · Anaheim, US
• Jill DuBois-Bousamra · Fishers, US
• Eric R. Diebold · Fishers, US
• Myron Rapkin · Indianapolis, US
• Mark T. Skarstedt · Elkhart, US

Key dates

• Filing date: Jan 3, 1991
• Grant date: Nov 9, 1993
• Expiry date: Jan 3, 2011

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/805
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Non-aqueous, polymeric reagent film compositions for use with analytical test devices of the dry chemistry type are described. The film compositions are prepared from an organic solution of a copolymer formed by interaction of at least two monomers wherein PA0 (a) the first monomer is a hydroxylated acrylate of the general formula ##STR1## where R1 is hydrogen or, more preferably, methyl, and R2 is a hydroxyalkyl group having from 1 to 5 carbon atoms, and PA0 (b) the second monomer is a neutral acrylate of the general formula ##STR2## where R1 is hydrogen or, more preferably, methyl, and R3 is a substituted or unsubstituted alkyl group having from 1 to 8 carbon atoms. Preferably, a third monomer is also included which is an amine-containing acrylate of the general formula ##STR3## where R1 is hydrogen or, more preferably, methyl, and R4 is a substituted or unsubstituted aminoalkyl or glycidyl group having from 1 to 5 carbon atoms or a surfactant polyethylene glycol group having from 10 to 30 carbon atoms. The film further comprises at least one enzyme in powder form dispersed therein and, optionally, a chromogenic indicator. The film composition is especially useful for determining…