Enzyme-amplified lanthanide chelate luminescence
US5262299A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Nov 13, 1990 |
| Grant date | Nov 16, 1993 |
| Priority date | — |
| Expiry date | Nov 13, 2010 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/13
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A method and compounds useful for this method are described for enzyme-amplified signal detection in analytical assays requiring extremely high detection sensitivity which uses a substrate capable of being transformed by an enzyme from a compound which does not form a luminescent lanthanide chelate into a product which forms a luminescent lanthanide chelate. The method in which a substrate not capable of forming a highly luminescent lanthanide chelate is enzymatically altered to produce a product which forms a highly luminescent lanthanide chelate and hence is particularly useful in time-resolved luminescence analysis as required in many different heterogeneous or homogeneous assay formats is described herein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.