Methods to clone polyA mRNA
US5262311A · kind A · utility
123Cited by
1References
18Claims
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Key dates
| Filing date | Mar 11, 1992 |
| Grant date | Nov 16, 1993 |
| Priority date | — |
| Expiry date | Mar 11, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for isolating mRNAs as cDNAs employs a polymerase amplification method using at least two oligodeoxynucleotide primers, one being short with arbitrary sequence and another being either short with arbitrary sequence or being capable of hybridizing to the region near the mRNA polyA tail. The oligodeoxynucleotide that is capable of hybridizing to the region near the polyA tail is used as a primer for reverse transcription of the mRNA and the resultant cDNA is amplified with a polymerase using both oligodeoxynucleotides as a primer set.
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