Isolated DNA encoding the SPHI restriction endonuclease and related methods for producing the same
US5262318A · kind A · utility
2Cited by
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7Claims
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Key dates
| Filing date | Aug 20, 1992 |
| Grant date | Nov 16, 1993 |
| Priority date | — |
| Expiry date | Aug 20, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention is directed to a method for cloning and producing the SphI restriction endonuclease by 1) introducing the restriction endonuclease gene from Streptomyces phaeochromogenes into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the SphI restriction endonuclease activity, and 3) purifying the SphI restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the SphI restriction endonuclease activity.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.