Method and reagents for detecting amphetamine and/or D-methamphetamine in biological samples
US5262333A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 12, 1992 |
| Grant date | Nov 16, 1993 |
| Priority date | — |
| Expiry date | Jun 12, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/542
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This disclosure relates to a method and reagents for determining amphetamine and d-methamphetamine in a biological fluid, such as urine. In particular, this disclosure relates to improvements in a fluorescence polarization immunoassay procedure for determining the presence of amphetamine and d-methamphetamine in a single assay and to a novel class of tracer compounds employed as reagents in such procedures. The procedure described includes pretreatment of the biological sample to eliminate cross reactants such as .beta.-hydroxyphenethylamine by preincubating the sample solely with an aqueous periodate solution having a pH from about 4.0 to about 7.5 without adjustment to an alkaline pH, and contacting the sample with riboflavin binding protein to reduce interference from fluorescent components in the sample. The procedure also maintains the cross reactivity of the immunoassay for tyramine at about 0.4% and for 1-methamphetamine below about 5.1% and eliminates the necessity of using controlled substances as starting materials.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.