Capillary zone electrophoretic analysis of isoenzymes
US5264095A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jun 12, 1992 |
| Grant date | Nov 23, 1993 |
| Priority date | — |
| Expiry date | Jun 12, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44747
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein is a methodology for analyzing isoenzymes using capillary zone electrophoresis ("CZE") techniques. Briefly, an isoenzyme-containing sample and a substrate capable of being catalyzed by said isoenzyme into a reaction product are introduced into a capillary column comprising a buffer. Most preferably, the buffer contains the substrate prior to introduction of the sample into such substrate-buffer. CZE separation techniques are applied to the column such that the isoenzymes are separated from each other into discrete zones. The separation techniques are terminated such that product is rapidly generated by the catalytic conversion of substrate by the isoenzymes, and accumulated, within each discrete zone, followed by detection of product. Information regarding the relative distribution of the isoenzymes can be derived from the relative distribution of the product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.