Method for the separation, identification and quantification of isoenzymes and isoforms of alkaline phosphatase
US5264098A · kind A · utility
8Cited by
1References
22Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jan 15, 1992 |
| Grant date | Nov 23, 1993 |
| Priority date | — |
| Expiry date | Jan 15, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/447
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention essentially relates to a method for the separation, identification and quantification of the isoenzymes and isoforms of alkaline phosphatase in samples of physiological fluid or tissue extracts, in which method a buffer, at least one nonionic detergent and at least one anionic detergent are applied to a support medium. Hepatic, osseous, placental, macromolecular and intestinal isoenzymes and isoenzymes bound to LpX, in particular, may be detected reliably while avoiding, as far as possible, the use of confirmation tests.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.