Process for hemoglobin extraction and purification
US5264555A · kind A · utility
45Cited by
13References
64Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jul 14, 1992 |
| Grant date | Nov 23, 1993 |
| Priority date | — |
| Expiry date | Jul 14, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K14/805
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods are disclosed for separating hemoglobin from erythrocytes by contacting erythrocytes with a hypotonic buffer solution at a rate sufficient to render the release of hemoglobin from said erythrocytes without significant lysis. The hemoglobin is then separated from the erythrocytes. Methods are also disclosed for purifying hemoglobin solutions of DNA, endotoxins and phospholipids by contacting the hemoglobin solutions with an anion exchange medium.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.