Process for producing proteins using gram negative host cells

US5268270A · kind A · utility

Assignee

• Max-Planck-Gesellschaft zur Föderung der Wissenschaften e.V. · US

Inventors

• Thomas F. Meyer · Tübingen, DE
• ROMAN HALTER · Tübingen, DE
• Johannes Pohlner · Tübingen, DE

Key dates

• Filing date: Feb 29, 1988
• Grant date: Dec 7, 1993
• Expiry date: Feb 29, 2008

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/50
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

For the gene-technological production of proteins, a vector is introduced to gram-negative host cells which contains at least one gene coding translated. For the extracellular obtaining of the proteins its coding gene is so inserted into a vector which contains the IgA-protease precursor gene from micro-organisms of the genus Neisseria that the coding gene is positioned within the sequence of the IgA-protease precursor gene. A plasmid especially suitable for this purpose is the plasmid pIP100 contained in E. coli DSM 3775.