Method for immobilizing biocatalyst in granular form with a photo-crosslinkable resin
US5268286A · kind A · utility
Assignees
Inventors
Key dates
| Filing date | Mar 6, 1991 |
| Grant date | Dec 7, 1993 |
| Priority date | — |
| Expiry date | Mar 6, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N11/098
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A biocatalyst is immobilized with a graft product of a polymer and saponified polyvinyl acetate containing a stilbazolium group as a photo-crosslinking group. The polymer is preferably gelatin, collagen, starch, cellulose, gum arabic, tragacanth gum, carrageenan, mannan, dextrin or alginic acid. The graft product is prepared by bonding the polymer to the polyvinyl acetate, either directly through functional groups of the polymer and polyvinyl acetate or through a crosslinking agent. The polymer provides affinity for the biocatalyst and by grafting the polymer to the saponified polyvinyl acetate, damage to cells upon immobilization is reduced. A liquid composition containing a biocatalyst and the graft product is added to an aqueous solution of an inorganic salt or an organic salt to form a granular gel and the gel is irradiated with actinic rays to cure the gel.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.