Thermostable ribonuclease H
US5268289A · kind A · utility
15Cited by
2References
3Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 27, 1991 |
| Grant date | Dec 7, 1993 |
| Priority date | — |
| Expiry date | Dec 27, 2011 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N9/22
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A substantially pure preparation of thermostable RNase H is disclosed. In one preferable form of the invention, the RNase H is capable of biological activity after incubation at temperatures equal to or greater than 70.degree. C. for at least ten minutes. In another preferable form of the invention, the purification is from Thermus thermophilus or a closely related organism. The present invention is also a method of digesting RNA polymers that are in duplex form with a DNA molecule. The method comprises exposing the duplex to the isolated thermostable RNase H.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.