Preparation of a solid phase matrix containing a bound specific binding pair

US5268306A · kind A · utility

Assignee

• Boehringer Mannheim GmbH · DE

Inventors

• Michael Berger · Sharon, US
• Arno Deger · Penzberg, DE
• Josef Maier · Eichstätt, DE

Key dates

• Filing date: Dec 23, 1991
• Grant date: Dec 7, 1993
• Expiry date: Dec 23, 2011

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S530/816
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A specific binding pair is bound to an insoluble carrier for use in determining an analyte such as in an immunoassay. The carrier is coated with a first polymer containing a protein polymer having a molecular weight of at least about 20,000 and molecules of a first member of a specific binding pair. A second polymer containing a second member of the specific binding pair is bound to the first member on the carrier by binding of the first and second members of the specific binding pair. The first polymer is preferably more hydrophobic than the second polymer. The protein polymer can be prepared by cross-linking hydrophobic protein molecules of 10,000 to 700,000 molecular weight with a bifunctional or polyfunctional compound to obtain a protein polymer of 200,000 to 20,000,000 molecular weight. The second polymer can be the second member of the specific binding pair or the second member cross-linked with a linker or the second member cross-linked to a hydrophobic protein.