Methods for identifying nucleic acid ligands
US5270163A · kind A · utility
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21Claims
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Key dates
| Filing date | Aug 17, 1992 |
| Grant date | Dec 14, 1993 |
| Priority date | — |
| Expiry date | Aug 17, 2012 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/974
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The SELEX (Systematic Evolution of Ligands by EXponential Enrichment) method is disclosed for the identification of nucleic acid ligands. A candidate mixture of single stranded nucleic acids having regions of randomized sequence is contacted with a target compound and those nucleic acids having an increased affinity to the target are partitioned from the remainder of the candidate mixture. The partitioned nucleic acids are amplified to yield a ligand enriched mixture.
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