Assay by enzyme-catalyzed isotopic exchange
US5272054A · kind A · utility
5Cited by
10References
50Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Mar 26, 1992 |
| Grant date | Dec 21, 1993 |
| Priority date | — |
| Expiry date | Mar 26, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/804
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of assay for isotopically exchangeable analytes is disclosed. Analytes are labeled by enzymatic exchange of a hydrogen atom of the analyte and a deuterium or tritium atom. Preferably, analytes are labeled by reaction with an oxidant, a reducing agent which contains a deuterium or tritium atom, and an enzyme capable of catalyzing the reversible exchange of a hydrogen atom between the analyte, the oxidant, and the reducing agent. Kits for conveniently performing the assay methods are also disclosed.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.