Method for the modification of the expression characteristics of an endogenous gene of a given cell line
US5272071A · kind A · utility
Assignee
Inventor
Key dates
| Filing date | May 28, 1992 |
| Grant date | Dec 21, 1993 |
| Priority date | — |
| Expiry date | May 28, 2012 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/907
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Normally transcriptionally silent genes in a cell line or microorganism may be activated for expression by inserting a DNA regulatory element which is capable of promoting the expression of a normally expressed gene product in that cell or which is promiscuous, the regulatory element being inserted so as to be operatively linked with the normally silent gene in question. The insertion is accomplished by means of homologous recombination by creating a DNA construct including a segment having a DNA segment of the normally silent gene (targeting DNA) and the DNA regulatory element to induce gene transcription. The technique is also used to modify the expression characteristics of any endogenous gene of a given cell line or microorganism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.