Reagents and methods for the determination of glycohydrolytic enzymes
US5272260A · kind A · utility
Assignee
Inventors
Key dates
| Filing date | Jan 29, 1992 |
| Grant date | Dec 21, 1993 |
| Priority date | — |
| Expiry date | Jan 29, 2012 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S436/808
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Novel pyrene-trisulfonate derivatives and the use thereof in a method for the determination of glycohydrolytic enzyme activity are provided. The method comprises the steps of (a) forming a test solution comprising a test sample containing the glycohydrolytic enzyme and a pyrene-trisulfonate derivative of the present invention, wherein the derivative is hydrolyzed by the glycohydrolytic enzyme to result in the formation of free 8-hydroxy-1,3,6-pyrene trisulfonate as a function of, and which can be correlated to, the amount of the glycohydrolytic enzyme present in the test sample, and (b) measuring and correlating either the intensity of fluorescence, or the optical density, of the test solution to the presence or amount of the glycohydrolytic enzyme in the test sample. A preferred pyrene-trisulfonate derivative is pyrene-1,3,6-trisulfonic acid) 8-.beta. -D-glucuronide for the determination of .beta.-D-glucuronidase for the diagnosis of periodontal disease.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.